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NCBI Activity: Elizabeth & Dmitriy; Hsp 70

Group: Elizabeth & Dmitriy

Topic: Gene Conversion and GC content in Hsp 70 (Heat Shock Protein)

Introduction:

HSP70  is a highly conserved family of ubiquitous chaperon proteins. This study used several HSP70 proteins with very high similarity in the amino acid sequence but in contrast, great variance in the codons which code for the amino acids.

This paper demonstrated that the different codons for the same amino acid can up regulate or down regulate Hsp 70 expression. Specifically, greater GC content at the third position of the codon increases mRNA levels of Hsp 70. The increase in mRNA levels cannon be attributed to a change in mRNA stability because the stability of the different mRNA was virtually identical. The significance of this study is that it shows that mammals have selectively evolved different DNA sequences, without changing the protein, to have greater control of gene regulation.

Background:

Hsp 70 has a weight of 70 kilodaltons and plays a chaperoning and  housekeeping role. During periods of stress such as heat and specifically exposure to heavy metals the levels of Hsp 70 will jump in an organism.

References:

GC-Content Evolution in Mammalian Hsp70
Grzegorz Kudla*, Aleksandra Helwak{dagger},* and Leszek Lipinski{dagger},*
Mol. Biol. Evol. 21(7):1438-1444. 2004
DOI: 10.1093/molbev/msh146

The hsp70 family of molecular chaperones.Box
Molecular Biology of the Cell -> Basic Genetic Mechanisms -> How Cells Read the Genome: From DNA to Protein -> From RNA to Protein

High guanine and cytosine content increases mRNA levels in mammalian cells.
Grzegorz Kudla et al.
PLoS biology 4 (6), e180 (Jun 2006)
info:pmid/16700628 | info:doi/10.1371/journal.pbio.0040180

We used google alert to track any information or citations that appear on the internet with the title from the paper.

We learned how to set up a google alert, but we would like to figure out how to set up an alert using the actual paper not just the title.

Another good trick is using Connotea to transfer a paper between computers.

We would like to find a site that created a paper citation map.

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