Categories

Archives

A sample text widget

Etiam pulvinar consectetur dolor sed malesuada. Ut convallis euismod dolor nec pretium. Nunc ut tristique massa.

Nam sodales mi vitae dolor ullamcorper et vulputate enim accumsan. Morbi orci magna, tincidunt vitae molestie nec, molestie at mi. Nulla nulla lorem, suscipit in posuere in, interdum non magna.

Jennifer and Ayla: GFP

Green Fluorescent Protein (GFP)

It’s a protein that glows green when exposed to blue light.  It comes from the jellyfish Aequorea victoria.  It is often used in experiments as a reporter gene.  We chose it because it GLOWS!!!

PBD ID: 2g6e

Sources:
http://www.pdb.org/pdb/explore.do?structureId=2G6E#
http://www.pdb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb42_1.html

Looked at chain A in jmol

Species source Aequoria victoria (jellyfish), expressed in E. coli

If we could have gotten the ribbon structure to display (it was just showing us the space-fill model for some reason), we would have expected to see a sort of barrel shape in jmol like we saw on the pbd page.

In the Consurf you can see the surface on one end is highly conserved.  We would like to know if the conserved portion has anything to do with the fluorescence function of the protein.

gfpjmol

gfppdb

Different fluorescent proteins growing in bacteria

Different fluorescent proteins growing in bacteria

Comments are closed.