Green Fluorescent Protein (GFP)
It’s a protein that glows green when exposed to blue light. It comes from the jellyfish Aequorea victoria. It is often used in experiments as a reporter gene. We chose it because it GLOWS!!!
PBD ID: 2g6e
Sources:
http://www.pdb.org/pdb/explore.do?structureId=2G6E#
http://www.pdb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb42_1.html
Looked at chain A in jmol
Species source Aequoria victoria (jellyfish), expressed in E. coli
If we could have gotten the ribbon structure to display (it was just showing us the space-fill model for some reason), we would have expected to see a sort of barrel shape in jmol like we saw on the pbd page.
In the Consurf you can see the surface on one end is highly conserved. We would like to know if the conserved portion has anything to do with the fluorescence function of the protein.
Different fluorescent proteins growing in bacteria