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Alcohol Dehydrogenase

Dylan, Ellen, Stephen, and Jerreme

Alcohol Dehydrogenase

Alcohol dehydrogenase (ADH) is an enzyme that catalyzes the conversion of alcohols to aldehydes or ketones and a reduction of NAD+ to NADH.  ADH is advantageous to humans because it prevents alcohol toxicity by breaking down alcohol. ADH oxidizes only secondary and tertiary alcohols to aldehydes and ketones with the use of a coenzyme.

Reference:

Substitutions in the Flexible Loop of Horse Liver Alcohol Dehydrogenase Hinder the Conformational Change and Unmask Hydrogen Transfer
Ramaswamy, S.,   Park, D.H.,   Plapp, B.V.
(1999) Biochemistry 38: 13951-13959

A total of 190 sequences were used for the alignment including Mouse, Horse, Rat, Bovine, E.coli.

We compared side chain A of the protein and found the external regions to be highly variable with the interior regions exhibiting more conserved behavior. The ligand binding regions were also highly conserved.

Is the active site highly conserved?pbb_protein_adh5_image1

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