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Buffer Preparation

This workbook calculates the necessary components to create a buffer solution. The workbook will allow the user to create a buffer in two ways. A user can select an acid and its conjugate base or select an acid or base and combine it with a strong base or strong acid. Users will input the desired pH, concentration and volume of the solution and the workbook will output a recipe for a buffer solution using the Henderson-Hasselbalch equation.


John R. Jungck, Beloit College
Annelise L Myers, Beloit College
Stephanie Gage, Beloit College

Published by: BioQUEST Curriculum Consortium

OS: all

User Manuals and Curricular Materials
Popular Text Citations

Moore, J.W., C.L. Stanitski and P.C. Jurs, (2002). Chemistry The Molecular Science. Harcourt College Publishers, Orlando

Jack, R. Cecil. (1995) Basic Biochemical Laboratory Procedures and Computing. Oxford University Press, New York

Harris, D.C., (1987). Quantitative Chemical Analysis. W.H. Freeman and Company, New York. Second Edition.

Research Articles

Good, N.E.; Wingset, G.D.; Winter, W.; Connolly, T.N.; Izawa, S.; Singh, R.M.M. (1966) Hydrogen Ion Buffers for Biological Research. Biochemistry, 5:467-477.

Po, H.N.; Senozan, H.M. (2001) The Henderson-Hasselbalch Equation: Its History and Limitations. Journal of Chemical Education, 78: 1499-1503.

Tutorial & Background materials

Martin Chaplin. Biological Chemistry

“Chemistry and Background” (2003) . “ChemLab Darthmouth College. Trustees of Darthmouth College. Accessed 9 June 2005.

Jungck John R., Myers Annelise L, Gage Stephanie () Buffer Preparation. A module of the Biological ESTEEM Collection, published by the BioQUEST Curriculum Consortium. URL: