This project was prepared as part of a BioQUEST faculty development workshop entitled Numbers Count Workshop at Agnes Scott College in January 2009. The BioQUEST Curriculum Consortium is committed to the reform of undergraduate biology instruction through an emphasis on engaging students in realistic scientific practices. This approach is sometimes characterized as an inquiry driven approach and is captured in BioQUEST's three P's (problem-posing, problem-solving, and peer-persuasion). As part of this workshop groups of faculty were encouraged to initiate innovative curricular projects. We are sharing these works in progress in the hope that they will stimulate further exploration, collaboration and development. Please see the following links for additional information:

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Fluorescent Redistribution after Photobleaching.
Authors          Audiences          Overview           Materials          Resources           Future Directions




Possible Audiences:

Cell Biology students: undergradute and graduate  


Brief Overview:

Fluorescent redistribution after photobleaching (FRAP) is a common approach used to study the mobility of molecules within the cell. Using this method, researchers are able to characterize the mobile fraction of fluorescent molecules and the diffusion coefficient.  


Project Materials:

This data set was collected by Ann Cowan using the Microscope Facilities of the Center for Cell Analysis and Modeling at University of Connecticut Health Center ( Additional materials associated with this data set include an animation describing the FRAP process and exercises for determining properties of the APC molecule examined and for modeling FRAP experiments. See FRAP Tutorial at  


Resources and References:

Image intensity values collected from image data set available from UCHC CCAM. For additional information regarding use of FRAP data and lessons in classrooms contact  


Future Directions:

We are looking to use this data and related resources to introduce students to modeling and quantitative cell biology.