This project was prepared as part of a BioQUEST faculty development workshop entitled Bioinformatics Education Dissemination: Reaching Out, Connecting, and Knitting-together at University of Texas at Austin in May 2005. The BioQUEST Curriculum Consortium is committed to the reform of undergraduate biology instruction through an emphasis on engaging students in realistic scientific practices. This approach is sometimes characterized as an inquiry driven approach and is captured in BioQUEST's three P's (problem-posing, problem-solving, and peer-persuasion). As part of this workshop groups of faculty were encouraged to initiate innovative curricular projects. We are sharing these works in progress in the hope that they will stimulate further exploration, collaboration and development. Please see the following links for additional information:

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PFK Through The Ages
Authors          Audiences          Overview           Materials          Resources           Future Directions



Janet Baker
Middlesex County College

Jonathan Atwater
Southwestern College

Chitra Chandrasekaran
Texas Wesleyan University

Lizette Santos-Santori
Pontifical Catholic University of Puerto Rico


Possible Audiences:

Undergraduate General Biology, Cell Biology, Biochemistry  


Brief Overview:

The primary goal of this project to put metabolism in an evolutionary perspective by doing phylogenetic analyses among chosen taxonomic groups for ATP-dependent phosphofructokinase.  


Project Materials:

Data base file of amino acid sequences for phosphofructokinase for selected organisms  


Resources and References:

Biology Workbench
Protein Explorer  


Future Directions:

Include more organisms in the study. Use amino acid sequence alignment to examine protein structure at a three dimensional level (Protein Explorer). Examine the evolution of active sites and allosteric regulatory sites of phosphofructokinase Establish evolutionary connections by phylogenetic analyses.  



- PFK1.ppt