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Sequence It!

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SequenceIt! is a simulation program that allows you to experience the art and logic of protein sequencing through experimentation.

Your objective is to deduce the sequence of this polypeptide using many of the tools available to a practicing protein chemist.

You specify the operations and the order of their application. Success in determining the protein sequence depends on your understanding of the experimental procedures and on the logic you use in executing those procedures. Operations available range from simple cleavage reactions (enzymatic and chemical) which break a polypeptide into smaller fragments to complex sequential analyses (e.g., Edman degradation) that provide sequence information.