Instructions for translation of the nucleic acid coding sequence for   the Hemagglutinin gene

Step 1:  Open a browser and type in the URL address:  workbench.sdsc.edu

Step 2:  Set up your free account

Step 3:  Enter Biology Workbench and click on the 'session tools' button and select the 'run' button

Step 4:  Select "start a new session" from the menu and select the 'run' button

Step 5:  In the session description box, give the new session a name e.g. Hemagglutinin and select the 'start new session' button

Step 6: select the 'Nucleic tools' button, then Select Ndjinn - multiple database search and hit the 'run' button (see arrows)

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Step 7:  Enter the assession number indicated by the arrow, scroll down to find the GenBank Viral Sequences (see next diagram) and then select the 'search' button

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Step 8:  Select GBVRL:31871813 then the 'Import Sequence' button

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Step 9:  Now hit the 'run' button.

Step 10:  Select SIXFRAME from the scroll menu, Check the Influenza A box and hit the 'run' button

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Step 11:  Hit 'Submit' at bottom of the page

  
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Scroll through frames 1 through to six and decide which frame is showing the correct translation of our gene.  Remeber, the protein sequence requires an M for start and only one stop codon.