BioQUEST Curriculum Consortium
LifeLines OnLine
National Science Foundation
Howard Hughes Medical Institute

Workshop Announcement
and Travel

Prion biology problem space: Mad cows, itchy sheep and protein structure
Authors          Audiences          Overview           Materials          Resources           Future Directions



Srebrenka Robic
Agnes Scott College

Maryam Qureshi
Boston University


Possible Audiences:

Tools presented in the problem space can be used for in-class projects in introductory biology classes. More complex questions could be carried from introductory biology to more advanced biochemistry, molecular biology and evolution classes. Undergraduate students could also use this as a starting point to develop independent research projects.  


Brief Overview

Prions are infectious protein particles associated with various neurodegenerative diseases in humans and animals. This problem space introduces basic skills in protein structure and sequence exploration that can be used to analyze some unusual properties of prions, and develop testable hypotheses that can be explored through these tools. Students will be challenged to use a systems-biology approach to link structure, evolution and function of proteins.  


Project Materials

Power Point Presentation: Prion Problem Space: Mad cows, itchy sheep and protein structure?  


Resources and References

1. GeneDoc software for sequence analysis from
2. CN3D structure visualization and analysis software from NCBI
3. ProParam tool for calculation physical parameters of protein sequences
4. - online collection of various experimental results, visualization models, and stories about BSE (NOT peer-reviewed, but with links to many published articles. Also not updated since April 2001.)
5. Abstracts and references for articles about yeast prions.
6.Helical wheel creation software
7.PrP and Doppel sequences
8.List of some (Q/N) repeat-rich proteins  


Future Directions

We plan to impliment this activity in two classrooms this academic year.  



- PrionPPSatBlack.ppt