Wnt protein
By Xiaomin and Jianzhuang
Introduction: Wnt proteins form a family of highly conserved secreted signaling molecules
that regulate cell-to-cell interactions during embryogenesis. Wnt proteins bind to
receptors of the Frizzled and LRP families on the cell surface. Mutations in Wnt genes or
Wnt pathway components lead to specific developmental defects, while various human
diseases, including cancer, are caused by abnormal Wnt signaling.
Bibliographic reference
The Wnt Homepage [http://www.stanford.edu/~rnusse/wntwindow.html]
Cohen ED, Tian Y, Morrisey EE. Wnt signaling: an essential regulator of cardiovascular
differentiation, morphogenesis and progenitor self-renewal.
Development. 2008 Mar;135(5):789-98.
Species used in the alignment:
CLUSTAL W (1.81) multiple sequence alignment
Q8VEJ3 ——KGQEGESCLRTSDCGPGLCCARHFWTKICKPVLREGQVCSRRGHKDTAQAPEIF
Q9UBT3 ——KGQEGESCLRTFDCGPGLCCARHFWTKICKPVLLEGQVCSRRGHKDTAQAPEIF
Q9UBU2 –MSHIKGHEGDPCLRSSDCIEGFCCARHFWTKICKPVLHQGEVCTKQ-RKKGSHGLEIF
2jtk_A GSMPHIKGHEGDPCLRSSDCIDGFCCARHFWTKICKPVLHQGEVCTKQ-RKKGSHGLEIF
Q9QYZ8 –MPHIKGHEGDPCLRSSDCIDGFCCARHFWTKICKPVLHQGEVCTKQ-RKKGSHGLEIF
O94907 –MYHTKGQEGSVCLRSSDCASGLCCARHFWSKICKPVLKEGQVCTKH-RRKGSHGLEIF
O54908 —-HTKGQEGSVCLRSSDCAAGLCCARHFWSKICKPVLKEGQVCTKH-KRKGSHGLEIF
**:**. ***: ** *:*******:******* :*:**:.. … ::. ***
Q8VEJ3 QRCDCGPGLTCRSQVTSNRQH–SRLRVCQRI
Q9UBT3 QRCDCGPGLLCRSQLTSNRQH–ARLRVCQKI
Q9UBU2 QRCDCAKGLSCKVWKDATYSSK-ARLHVCQKI
2jtk_A QRCDCAKGLSCKVWKDATYSSK-ARLHVCQKI
Q9QYZ8 QRCDCAKGLSCKVWKDATYSSK-ARLHVCQKI
O94907 QRCYCGEGLSCRIQKDHHQASNSSRLHTCQR-
O54908 QRCYCGEGLACRIQKDHHQASNSSRLHTCQR-
*** *. ** *. . :**..**.
image
interpretation of the results
The highly conserved domain are almost in the surface of three-dimension structure, which
are in purple color. The inner part of the protein are variable.
questions you are still working on
We can see large part of yellow color in the image. That means there are still some
insufficient data about the conservation of this protein. We need further research on the
yellow part.