This workbook calculates the necessary components to create a buffer solution. The workbook will allow the user to create a buffer in two ways. A user can select an acid and its conjugate base or select an acid or base and combine it with a strong base or strong acid. Users will input the desired pH, concentration and volume of the solution and the workbook will output a recipe for a buffer solution using the Henderson-Hasselbalch equation.

Henderson-Hasselbalch

Henderson-Hasselbalch

Hasselbalch, K. A. 1916. Biochemische. Zeitschrift 78: 112-144.

Lawrence J. Henderson. 1908. Concerning the relationship between the strength of acids and their capacity to preserve neutrality. Am. J. Physiol. 21:173-179.

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Jack, R. Cecil. (1995) Basic Biochemical Laboratory Procedures and Computing. Oxford University Press, New York

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Good, N.E.; Wingset, G.D.; Winter, W.; Connolly, T.N.; Izawa, S.; Singh, R.M.M. (1966) Hydrogen Ion Buffers for Biological Research. Biochemistry, 5:467-477.

Po, H.N.; Senozan, H.M. (2001) The Henderson-Hasselbalch Equation: Its History and Limitations. Journal of Chemical Education, 78: 1499-1503.

Martin Chaplin. Biological Chemistry

“Chemistry and Background” (2003) . “ChemLab Darthmouth College. Trustees of Darthmouth College. Accessed 9 June 2005.