Sequence alignment

Consensus key (see documentation for details)

* - single, fully conserved residue

: - conservation of strong groups

. - conservation of weak groups

- no consensus

CLUSTAL W (1.8) multiple sequence alignment

GDF5_MOUSE MRLPKLLTLLLWHLAWLDLELICTVLGAPDLGQRTPGAKPGLTKAEAKERPPLARNVFRP

GDF5_HUMAN MRLPKLLTFLLWYLAWLDLEFICTVLGAPDLGQRPQGSRPGLAKAEAKERPPLARNVFRP

********:***:*******:*************. *::***:*****************

GDF5_MOUSE GGHIYGVGAT--NARAKGSSGQT----QAKKDEPRKMPPRSGGSETKPGPSSQTRQAAAR

GDF5_HUMAN GGHSYGGGATNANARAKGGTGQTGGLTQPKKDEPKKLPPRPGGPEPKPGHPPQTRQATAR

*** ** *** ******.:*** *.*****:*:***.**.*.*** ..*****:**

GDF5_MOUSE TVTPKGQLPGGKASSKAGSAPSSFLLKKTREPGTPREPKEPFRPPPITPHEYMLSLYRTL

GDF5_HUMAN TVTPKGQLPGGKAPPKAGSVPSSFLLKKAREPGPPREPKEPFRPPPITPHEYMLSLYRTL

*************..****.********:****.**************************

GDF5_MOUSE SDADRKGGNSSVKLEAGLANTITSFIDKGQDDRGPAVRKQRYVFDISALEKDGLLGAELR

GDF5_HUMAN SDADRKGGNSSVKLEAGLANTITSFIDKGQDDRGPVVRKQRYVFDISALEKDGLLGAELR

***********************************.************************

GDF5_MOUSE ILRKKPLDVAKPAVPSSGRVAQLKLSSCPSGRQPAALLDVRSVPGLDGSGWEVFDIWKLF

GDF5_HUMAN ILRKKPSDTAKPAVPRSRRAAQLKLSSCPSGRQPAALLDVRSVPGLDGSGWEVFDIWKLF

****** *.****** * *.****************************************

GDF5_MOUSE RNFKNSAQLCLELEAWERGRAVDLRGLGFERTARQVHEKALFLVFGRTKKRDLFFNEIKA

GDF5_HUMAN RNFKNSAQLCLELEAWERGRTVDLRGLGFDRAARQVHEKALFLVFGRTKKRDLFFNEIKA

********************:********:*:****************************

GDF5_MOUSE RSGQDDKTVYEYLFSQRRKRRAPLANRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIA

GDF5_HUMAN RSGQDDKTVYEYLFSQRRKRRAPSATRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIA

*********************** *.**********************************

GDF5_MOUSE PLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFID

GDF5_HUMAN PLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFID

************************************************************

GDF5_MOUSE SANNVVYKQYEDMVVESCGCR

GDF5_HUMAN SANNVVYKQYEDMVVESCGCR

*********************

Clustal W options and diagnostic messages

Alignment type: Protein Alignment order: aligned

Pairwise alignment parameters

Method: accurate

Matrix: Gonnet

Gap open pentalty: 10.00 Gap extension pentalty: 0.10

Multiple alignment parameters

Matrix: Gonnet Negative matrix?: no

Gap open pentalty: 10.00 Gap extension pentalty: 0.20

% identity for delay: 30 Residue-specific gap penalties: on

Penalize end gaps: on Hydrophylic gap penalties: on

Gap separation distance: 0 Hydrophylic residues: GPSNDQEKR

CLUSTAL W (1.8) Multiple Sequence Alignments

Sequence type explicitly set to Protein

Sequence format is Pearson

Sequence 1: GDF5_MOUSE 495 aa

Sequence 2: GDF5_HUMAN 501 aa

Start of Pairwise alignments

Aligning...

Sequences (1:2) Aligned. Score: 91

Guide tree file created: [cw27117.dnd]

Start of Multiple Alignment

There are 1 groups

Aligning...

Group 1: Sequences: 2 Score:10367

Alignment Score 2900

CLUSTAL-Alignment file created [cw27117.aln]

Clustal W dendogram

(1_GDF5_MOUSE:0.04242,0_GDF5_HUMAN:0.04242);