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by Scott Cooper (University of WisconsinóLa Crosse, email@example.com)
What is a primer?
A primer is a short synthetic oligonucleotide
which is used in many molecular techniques from PCR
to DNA sequencing. These primers are
designed to have a sequence which is the reverse complement of a region
of template or target DNA to which we wish the primer to anneal.
Analysis of primer sequences
When designing primers for PCR, sequencing or mutagenesis it is often necessary to make predictions about these primers, for example melting temperature (Tm) and propensity to form dimers with itself or other primers in the reaction. The following program will perform these calculations on any primer sequence or pair.
Some thoughts on designing primers.
from Innis and Gelfand,1991)