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The objective was to "develop" a heat resistant amylase with the starch processing power of a corn amylase and the heat resistant qualities of a heat resistant amylase.
The first step was to find the two "base" amylases whose crystal structure was available via PDB. With the use of google, help from Ethel, and little luck, the two candidates were identified: 1VJS (the heat resistant amylase) and 1IZK ( the corn amylase).
The next step was to analyze the structures of both the enzymes on the ConSerf server along with similar proteins (as found on BLAST) to identify the conserved areas.
The final step was to identify regions on either enzyme where modifications could be performed to achieve the desired properties.
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