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From 1991 until 1993 the Biosphere system supported people, plants, and animals. Experiments offered the opportunity for tracking in great detail the interactions among land and water ecosystems and the atmosphere.

SimBio2 offers several scenarios in which students can generate simulation graphs using actual data from the Biosphere 2 experiment. Students can explore some of the problems that affected Biosphere 2 such as higher levels of carbon dioxide than expected and the resulting decrease oxygen. Students can also create their own models and experiment with alternative strategies.