RateIt! allows you to experience the art of enzyme kinetics through experimentation. The program can select a set of solutions containing enzymes, substrates, inhibitors, etc. Your objective is to deduce and characterize the kinetic properties of an enzyme-catalyzed reaction using all the tools available to a enzymologist. You can also define the kinetic properties of an enzyme and then perform experiments to see if the results are consistent with your defined properties.
Instead of being given operations and their results, as in a textbook, you specify the operations and the order of their application. Success in determining the properties of an enzyme-catalyzed reaction depends on your understanding of the experimental procedures and on the logic you use in executing those procedures. Operations available range from simple one substrate-one enzyme kinetics to complex reaction pathways involving inhibitors, products, and activators. The program replicates laboratory situations (with associated errors and ambiguities) and places limits on the amount of material available for analysis.
A RateIt! experiment begins with an ice bucket containing enzymes and substrates. The Experiment Condition windows, shown above, shows you the current values of some of the experimental parameters and allows you to modify them. Clicking on the OK button will display the Kinetic Experiment Summary window, similar to the figure below.
Macintosh or Power Macintosh